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Re: accurate total fat from FAMES.
Posted: Wed Mar 20, 2013 5:41 pm
by lgchrom
Just because the petro ether is very flamable and my lab is all one room including the flash point tester on the other side with an open flame.
Aah, I see. Though, if properly cooled with water, the Soxhlet device should put out no fumes at all. But if you have open flame in the lab, better safe than sorry.
Re: accurate total fat from FAMES.
Posted: Wed Mar 20, 2013 7:46 pm
by Steve Reimer
And if you lose the cooling water to the soxhlets and all the solvent evaporates into the room...
Re: accurate total fat from FAMES.
Posted: Wed Mar 20, 2013 8:02 pm
by dblux_
And if you lose the cooling water to the soxhlets and all the solvent evaporates into the room...
Yes, accidents happen. Glass breaks sometimes even without obvious reasons.
These should be taken into account.
Re: accurate total fat from FAMES.
Posted: Wed Apr 03, 2013 11:06 pm
by MSCHemist
Well I've decided I am going to buy a soxhlet extractor and do the USDA-FSIS method. 3.5g sample with sand, dry in an oven, soxhlet extract with pet ether for min 80 cycles, boil off ether, dry remaining ether in oven and weigh and then take that for FAMES.
Re: accurate total fat from FAMES.
Posted: Thu Apr 04, 2013 1:53 am
by KM-USA
We'd have to have an expiration date for the sand.
Re: accurate total fat from FAMES.
Posted: Fri Apr 05, 2013 7:47 pm
by JGK
Normally I wouldn't mind soxlet but my lab is kind of limited with hood space and I have no water plumbing other than one sink and the dishwasher. I operate a small satellite lab and I try to make it into a swiss army knife lab where I can do as much as possible albeit not always the most efficient way. I have low sample load but high variety.
You might find this useful
http://www.radleys.com/pages/products/findenser.shtml