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Grob Test

Discussions about GC and other "gas phase" separation techniques.

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Grob Test

avatar
WK
Hi All,
I have just bought a grob test mix and the chromatography I get is good except the 2,3-butanediol. For this I get 2 peaks (the 1st is half the size of the 2nd). They both have the same mass spectra. All grob tests published have one peak for the 2,3-butanediol. Does anyone have the same problem? I am running a RTX5 both on MS and FID and the problem is the same. Injection split 100:1 1mlmin helium 0.1ul inj 250degC.
Best Regards
WK

avatar
MikeD
WK

They are the two diastereoisomers. To get one peak you either need to obtain one of the diastereoisomers or the meso- form. I didn't bother - I just used the twin peaks as my column performance reference point.

avatar
MikeD
WK

I just realised you said you bought the mix. I made up my own Grob mix and could only blame myself. Where did you buy it ?
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