Calculating backflash

[pstaunton]

If I have sample that's diluted 1:1 with acetonitrile and my sample is mostly water based I obviously have to take both components into account when calculating potential back flash.

Sorry new to the topic so the question/solution is probably simple for you guys!

[Peter Apps]

Are you trying to estimate the vapour volume from a sample injection, so that you can select the correct inlet liner ? Just calculate for 1/2 ul of acetonitrile and 1/2 ul of water and add them. The GC manufacturers have tools for this on their websites. It is, at best, an inexact science.

Peter

[Bigbear]

Plus you will be limited as to the volume you can inject as well as lowering your injection temp. When I had to inject water into an Agilent 6890 using a liner ( internal volume 900ul)
I could only inject 0.5 ul and the injector was just above 100C.