Chromatography Forum

Hi ! As we know that RRF is the detector response of impurity relative to main compound at the same lambda & conc.
Now my question is that if the lambda of impurity & compound is same,but the purity of impurity is too much less, then what will be the RRF of impurity? Is it almost one because the lambda is same or less then one because the purity is less?

I don't fully understand your question, but if you do not have a purity value for your impurity, then you cannot reliably calculate a RRF for this said impurity.

Thanks for reply!

We can't do RRF if we don't have purity value of Impurity that's true.But the question is if the purity of impurity is less (i.e. around 50-60%) then the RRF is less than 1 or almost 1, like when the same impurity is more than 90% (assumes that lambda is same for compound & impurity)

Assuming that the impurities in the impurity have no absorbance at the wavelength in question, and assuming you make no correction for the purity, then the response factor of the impurity will be underestimated (e.g., a 1 ng injection of the impurity will generate a peak that is smaller than it should be).