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How facile is the conversion of CH3CN to acetamide in presence of acid acid (TFA)?
Since this rxn proceeds through the addition of water, would it not be avoided if neat TFA were added to pure acetonitrile?
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YesAnd the rest 60% is only water or only ACN which you use to do perform gradients from 0% to 60% ACN?
Just to clarify, you add an ampule of neat TFA to pure CH3CN, and experience no increase in background absorbance at 220 nm?It's an equilibrium reaction so you need a lot of water around to favor that transformation.
We usually just add a whole 1 mL ampule of TFA to the acetonitrile and have never had a problem.
We can't but add TFA directly to acetonitrile if use NP-HPLC, because water will be avoid in this system .I don't introduce 100% TFA. It is 0.25% aqueous TFA, and is pumped in at a constant 40% of the total mobile phase to get a final concentration of 0.1%
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