Chromatography Forum

We are trying to analyse Pentosan polysulphate sodium for average molecular weight by GPC. But we are not able get different molecular weight standrads required for calibration of GPC system. Is there any vendor from where these standards are available.

My second question is, is there any method for assay of pentosan polysulphate sodium?

I assume that you are asking about standards of the same polymer. Such standards are not likely to exist. However, you can calibrate your column(s) with related standards. For example, you can purchase polyacrylic acid standards from Waters. You can then express the molecular weight of your polymer in equivalents of the polyacrylic acid, or you can translate this molecular weight through a universal calibration curve. How easily this can be done depends on the information about your polymer or the equipment that you have.

As I remember right PSS can produce custom made polymer standards for GPC analysis. I assume that this will be expensive. Maybe it’s worth to contact them.

But instead of buying expensive standards, I would invest a bid more and go for a LS detector or Visco detector (or top of the bill GPC-MALDI). A much cheaper, and as precise, would be calibration with polyacrylic acid or pullulan. With Mark Houwink constants you could also get accurate molecular weight averages.