by
zlb215 » Wed Dec 17, 2008 5:31 am
I dissolve 50g ammonium acetate in 1L water.
As you said, when I mix ACN and buffer(3:1), the mixture divide into two layers, but the rate of layers is not the same as ACN :buffer(the layer underside is larger than upper).So I changed the proportion to 1:1, and got a pellucid mixture.However,when the room temperature changed from 25 to 8 centigrade, the mixture(1:1) separate into two layer again.
Can you explain this phenomenon for me? Thank you very much.
In addition, my hplc column's lifetime is very short(about 100 injections) using this mobile phase. After 100 injections, the peak become broad, then backpressure increase drastically.Did high concentration salt buffer cause that?