post column infusion

[parveenqa]

can you please guide me at post column infusion in LC-MS/MS
-why we have to do it?
- its importance
-advantages

[sassman]

You need to give more information. What is your analyte, mobile phase, what reagent are you adding post column, etc. Without knowing this we cannot help you.

[parveenqa]

thankyou for your interest
actually i want to know generally about post column infusion but i can give you all the details you asked
analyte: spironolactone
mobile phase: methanol:wter

[brewer]

Post column infusion is typically utilized to identify areas of ionization suppression in ESI mass spectrometry.

R King et al
j Am Soc Mass Spectrom 2000, 11, 942-950

[parveenqa]

You need to give more information. What is your analyte, mobile phase, what reagent are you adding post column, etc. Without knowing this we cannot help you.

thankyou for your interest
actually i want to know generally about post column infusion but i can give you all the details you asked
analyte: spironolactone
mobile phase: methanol:wter