can i analyse coloured compounds by HPLC

[parveenqa]

i want to kow whether i can carry out analysis of natural coloured compounds(carotenoids etc) by HPLC or it will effect column by some means???

[krickos]

Hi

Carotenoids is a HUGE group but a quick search in Journal of Chromatography B gave me some articles (see examples below), so obviously you can do it on LC at least some of them.


1.
Rapid quantitative determination of fat-soluble vitamins and coenzyme Q-10 in human serum by reversed phase ultra-high pressure liquid chromatography with UV detection
Journal of Chromatography B, In Press, Corrected Proof, Available online 14 November 2008
Ekaterina M. Paliakov, Brian S. Crow, Michael J. Bishop, Dean Norton, Joe George, J. Alexander Bralley

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2.
Effect of a traditional Chinese medicine preparation Xindi soft capsule on rat model of acute blood stasis: A urinary metabonomics study based on liquid chromatography–mass spectrometry
Journal of Chromatography B, Volume 873, Issue 2, 1 October 2008, Pages 151-158
Xinjie Zhao, Yi Zhang, Xianli Meng, Peiyuan Yin, Chong Deng, Jing Chen, Zhang Wang, Guowang Xu

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3.
Simultaneous measurement of retinol, α-tocopherol and six carotenoids in human plasma by using an isocratic reversed-phase HPLC method
Journal of Chromatography B, Volume 867, Issue 2, 15 May 2008, Pages 226-232
Jouni Karppi, Tarja Nurmi, Begoña Olmedilla-Alonso, Fernando Granado-Lorencio, Kristiina Nyyssönen

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4.
Quantification of fat-soluble vitamins in human breast milk by liquid chromatography–tandem mass spectrometry
Journal of Chromatography B, Volume 859, Issue 2, 15 November 2007, Pages 192-200
Maya Kamao, Naoko Tsugawa, Yoshitomo Suhara, Akimori Wada, Toshiyuki Mori, Kazuo Murata, Riichiro Nishino, Tetsuya Ukita, Kazuhiro Uenishi, Kiyoshi Tanaka, Toshio Okano

[krickos]

Hi again

Adding another artecle as I summarises a few analytical approches but also brings up potential stability issues, for instance after extraction into organic solvents.

19.
Carotenoids: separation methods applicable to biological samples
Journal of Chromatography B, Volume 781, Issues 1-2, 5 December 2002, Pages 393-418
Qing Su, Kevin G. Rowley, Nicholas D. H. Balazs

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[Bryan Evans]

Yes you can analyze these compounds by HPLC. Whether or not to
use RP or NP is usually dependant on sample solvent, detector, selectivity, ect. Below are links to some chromatograms:

Carotenoids on Cadenza CD-C18:
http://www.silvertonesciences.com/files/TI242E.pdf

Chlorophyll and carotenoid on Unison UK-C18:
http://www.silvertonesciences.com/files/TI228E.pdf

Fat soluble vitamins on Unison UK-Silica:
http://www.silvertonesciences.com/files/TI237E.pdf

Column lifetime is dependant on sample clean up methods.
You can also use a guard cartridge in front of the column.

[oscarBAL]

Hello Parveenga; what kind of sample you want to analyze?
there are a lot of information about it; I used to analyze pigments form algea; instead to go to the microscope I did extracted the pigment and went to the HPLC; this way I could to determinate presecen of certain tipes of algaes.

Regards.

Oscar

[Uwe Neue]

There is no problem with colored compounds... This is why they call it chromatography (chromos - color, graphein - writing).

[parveenqa]

Hi again

Adding another artecle as I summarises a few analytical approches but also brings up potential stability issues, for instance after extraction into organic solvents.

19.
Carotenoids: separation methods applicable to biological samples
Journal of Chromatography B, Volume 781, Issues 1-2, 5 December 2002, Pages 393-418
Qing Su, Kevin G. Rowley, Nicholas D. H. Balazs

Preview PDF (230 K) | Related Articles

Thankyou very much for your valuable information...

[parveenqa]

thankyou for all the answers that were really helpful