new to HPLC have some questions :)

[che313]

Dear all HPLC expert and user

I'm looking for utilizing liquid chromatography in our lab where our duty is for identifying drugs (tablets, powders, samples, etc..), detect drugs in body fluids; blood & urine.

My questions are:

* How can I build a HPLC system which satisfy my requireds. (i.e. best injector, type of pump, type of columns, best detector, etc...)
* Which books I have to read as a biggner?
* Which websites give me a better understanding for HPLC?


Best regards

[skunked_once]

How can I build a HPLC system which satisfy my requireds.

You need to identify your requirements. What are your analytes, types of samples, numbers of samples, etc. As far as which brand of instrument, which vendors serve your geographical area? Can you attend some meetings where you can talk to other chromatographers doing similar types of analyses? You need to find out all you can from vendors and the literature.

Which books I have to read as a biggner?
* Which websites give me a better understanding for HPLC?

Check out the sponsors of this web site. There is more than enough information to learn from. Good luck!

[Bruce Hamilton]

I would suggest that you locate a suitable HPLC training course, perhaps even some of the on-line ones offered at this site.

If you plan on analysing pharmaceuticals and metabolites, you really need to be discussing the best options with instrument manufacturers, as you may need software and systems that match regulatory compliance requirements.

The critical aspect would be the local technical support for the instruments. There's no point in purchasing an instrument if you can't obtain good local support, so you need to find out what other local HPLC users recommend as well-supported products..

Also, all of the major brands ( Agilent, Waters, Dionex etc ) offer support and training, so maybe you should ensure they include some relevant training cources as part of any hardware purchase.

Bruce Hamilton

[tom jupille]

http://www.lcresources.com/wiki/index65 ... Q:MoreInfo

[Ninocka]

Well, if there is someone who could answer me this:
when I have to set the wave length, there is
store signal, Bw Reference, Bw
what does this Bw, and Reference mean?



Most tnx in advance...

[JGK]

Well, if there is someone who could answer me this:
when I have to set the wave length, there is
store signal, Bw Reference, Bw
what does this Bw, and Reference mean?



Most tnx in advance...

Which instrument? Terminology will differ with each manufacturer

Have you read the User manual?

[Ninocka]

read it on the help menu, and there is no explanation, just an example...in ChemStation tutorial.
I use Agilent HPLC now.

[danko]

It stands for band width and the unit is typically nm. Sometimes it could be percentage.

Best Regards

P.S. Btw, your question is a bit off topic.

[Ninocka]

topic is: new to HPLC have some questions
so where else to put the question?

But, in the box "signal, Bw" I will put the length of interest, and what to put in the box "Reference, Bw". I could even turn it off, but I'd like to know for what it stands for?
In your answer was no explanation.

Tnx for trying...
Maybe you did not understand my question?

[danko]

Hi Ninocka,

topic is: new to HPLC have some questions
so where else to put the question?

In this case, it would have been most appropriate to initiate a new thread/topic. The topic name might have been; “detector wavelength setupâ€

[Ninocka]

That is the answer i was looking for, because i saw that many methods have Reference wavelength to 360 nm and its Band Width to 100 nm, but did not know why. How they get that numbers.
Tnx for clarification.

N.