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TFK as an ion pairing reagent
Posted: Thu Dec 11, 2008 9:37 pm
by adi.nu.r
Hello
I read an article in which they used TFK 100 mM as an ion pairing reagent.
I read about TFK and I dont think its the best thing to use in a RP HPLC system...
I wondered if anyone has an idea regarding another ion pairing reagent I can use?
Best regards
Posted: Thu Dec 11, 2008 9:56 pm
by sassman
What's TFK?
TFK - Trifluoromethylketone
Posted: Fri Dec 12, 2008 8:45 am
by adi.nu.r
Sorry about that.
Best regards.
Posted: Fri Dec 12, 2008 9:23 am
by HW Mueller
Maybe it forms a charge transfer complex with some (and only some) compounds??
My compound is 3-palmitoyl-sn-glycerol
Posted: Fri Dec 12, 2008 12:22 pm
by adi.nu.r
My compound is 3-palmitoyl-sn-glycerol
Its a monoglyceride.
Im looking for something to replace the TFK.
Thanks.
Posted: Fri Dec 12, 2008 2:58 pm
by HW Mueller
I don´t see any possibility for interaction here that could even remotely be called ionic. Maybe some hydrogen bonding from the OH?
There are a million methods for lipids of your type out there.
Posted: Fri Dec 12, 2008 5:43 pm
by sassman
Hmmm, still don't know what TFK is? As HWM said, your compound is not ionic so it shouldn't require an ion pairing reagent. I don't see why you would need any mobile phase additive for that matter. I would try running without the TFK. What kind of column do you plant to use?
Posted: Fri Dec 12, 2008 5:49 pm
by adi.nu.r
Actually, in the article I read, they said nothing about the part of TFK in the method. I asumed its there as an ion pairing reagent...
So should I ignore what they wrote in that article I read and try to do it in a different method?
Cause we already bought the column...
Best regards.
Posted: Fri Dec 12, 2008 5:52 pm
by adi.nu.r
The column we bought is Fortis H2o.
TFK - Trifluoromethylketone
Thanks.