Chromatography of Zwitterionic Buffers (MES, MOPS, etc.)

[Nalizer]

Hello:

I am looking for a simple method to analyze zwitterionic buffers, like MOPS and HEPES

http://en.wikipedia.org/wiki/MOPS

What are my choices? I have no retention on RP column even at very low organic (various pH)

[tom jupille]

That would be a classic application for ion-pair chromatography, or else one of the "mixed-mode) packings with embedded ion-exchange groups. Or, you could use good old ion-exchange (just run at a pH that suppresses one of your charged sites).

[Uwe Neue]

I would consider HILIC as well.

[depstein]

I found this article:

Analytical Chimica Acta 583 (2007) pp 124-127 where HEPES is the internal standard and they used an amide-80 column.

If you found any other solutions, I'd be interested in hearing about them.

[Vlad Orlovsky]

Here are few methods for several zwitter-ionic buffers:

http://www.sielc.com/compound_256.html

regards,

Vlad