Quantitation through LC-MS/MS

[subhrakanti]

Hi
For trace label analyte quantitation and confirmation by LCMSMS in a complex matrix quantitation and confirmation ion peak area ratio of targeted analyte is considered. how much deviation of this ratio is acceptable for an analyte from standard sample to matrix sample?

can you suggest me any literature reference for this?

[JGK]

What type of assay are you performing?

For Bioanalytical assays the FDA guidance is useful and gives you acceptance criteria for measurement accuracy

http://www.fda.gov/CDER/GUIDANCE/4252fnl.pdf

[MaryCarson]

Hi
For trace label analyte quantitation and confirmation by LCMSMS in a complex matrix quantitation and confirmation ion peak area ratio of targeted analyte is considered. how much deviation of this ratio is acceptable for an analyte from standard sample to matrix sample?

can you suggest me any literature reference for this?

For confirmation of identity in the case of US veterinary drug residues, see CVM Guidance 118:
http://www.fda.gov/cvm/Guidance/guide118.htm
For your example, the relevant text would be
"a. If a precursor ion selected by MSn is completely dissociated, and only two structurally-specific product ions are monitored in MSn+1, the relative abundance ratio should match the comparison standard within ± 10%."

EU criteria are slightly different; the permitted deviation depends on the relative abundance of the confirmatory ion. EU criteria can be found here:
http://faolex.fao.org/docs/pdf/eur49615.pdf
Look for Table 4 in section 2.3.3.2

Both of these sets of criteria are for fairly specific applications, and may not be appropriate for your application. I am not sure what you mean by "trace label analyte"--are you looking at a radioactivity-tagged compound?