TFK as an ion pairing reagent

[adi.nu.r]

Hello
I read an article in which they used TFK 100 mM as an ion pairing reagent.
I read about TFK and I dont think its the best thing to use in a RP HPLC system...

I wondered if anyone has an idea regarding another ion pairing reagent I can use?

Best regards

[sassman]

What's TFK?

[adi.nu.r]

Sorry about that.

Best regards.

[HW Mueller]

Maybe it forms a charge transfer complex with some (and only some) compounds??

[adi.nu.r]

My compound is 3-palmitoyl-sn-glycerol
Its a monoglyceride.

Im looking for something to replace the TFK.

Thanks.

[HW Mueller]

I don´t see any possibility for interaction here that could even remotely be called ionic. Maybe some hydrogen bonding from the OH?
There are a million methods for lipids of your type out there.

[sassman]

Hmmm, still don't know what TFK is? As HWM said, your compound is not ionic so it shouldn't require an ion pairing reagent. I don't see why you would need any mobile phase additive for that matter. I would try running without the TFK. What kind of column do you plant to use?

[adi.nu.r]

Actually, in the article I read, they said nothing about the part of TFK in the method. I asumed its there as an ion pairing reagent...

So should I ignore what they wrote in that article I read and try to do it in a different method?

Cause we already bought the column...

Best regards.

[adi.nu.r]

The column we bought is Fortis H2o.

TFK - Trifluoromethylketone

Thanks.