Chromatography Forum

Posted: **Wed Dec 10, 2008 7:05 am**

can you please guide me at post column infusion in LC-MS/MS
-why we have to do it?
- its importance
-advantages

Posted: **Wed Dec 10, 2008 8:27 pm**

You need to give more information. What is your analyte, mobile phase, what reagent are you adding post column, etc. Without knowing this we cannot help you.

Posted: **Tue Dec 16, 2008 10:35 am**

thankyou for your interest
actually i want to know generally about post column infusion but i can give you all the details you asked
analyte: spironolactone
mobile phase: methanol:wter

Posted: **Wed Dec 17, 2008 9:01 pm**

Post column infusion is typically utilized to identify areas of ionization suppression in ESI mass spectrometry.

R King et al
j Am Soc Mass Spectrom 2000, 11, 942-950

Posted: **Sat Dec 20, 2008 8:33 am**

You need to give more information. What is your analyte, mobile phase, what reagent are you adding post column, etc. Without knowing this we cannot help you.

thankyou for your interest
actually i want to know generally about post column infusion but i can give you all the details you asked
analyte: spironolactone
mobile phase: methanol:wter