Vitamins analysis of human samples

[James Ellis]

I am new to HPLC, but I would like to use this technology to assess the levels of water and fat-soluble vitamins from human samples. I would like to be able to identify/quantitate as many vitamins as possible from one method.

Does anyone have a suitable methodology or tips?

Thanks,
James.

[Bruce Hamilton]

As always, a good reference book is very helpful.

In this case,
Modern Chromatographic Analysis of Vitamins
Andre P. De Leenheer, Willy E. Lambert, Jan F. Van Bocxlaer
Marcel Dekker. 3rd Ed. ( 2000 ) ISBN: 0-8247-0316-2

This book will provide plenty of literature references, and points out many of the pitfalls of vitamin analysis.

Please keep having fun,

Bruce Hamilton

[Uwe Neue]

Look at the Waters website at the following brochure on Atlantis dC18. http://www.waters.com/webassets/cms/lib ... 0793en.pdf.

On page 7, it shows you a gradient separation of water-soluble and fat-soluble vitamins.

[Bryan Evans]

Below is LC-MS/MS application for water soluble vitamins on
Unison UK-C18:

http://www.silvertonesciences.com/files/TI312E.pdf

[James Ellis]

Thanks everyone. The links and references have been really useful.

James.

[oscarBAL]

Hello James; I am starting to read some articles because I am concediring to do my Thesis on a related thesis; I probably I am not so usefull but I advice you put atention to the sample preparation.
you can run fat and waters soluble vitamins in a single run; but this allways going to be limited for your ability to extract as much vitamins as you can in a single step.
This going to be my first experience with this kind of determination;In fact prior to the chromatographic method I am seeking a good sample preparation procedure.

Regards.

Oscar.