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Earliest Retention time

Posted: Thu Dec 04, 2008 10:48 pm
by Emiedoo
Hi all, I am using a Zorbax C18 1.8 um Agilent Eclipse Plus C18 column (2.1 × 50 mm). My earliest analyte eluting peak is at 0.82 min. My run time is 6.5 min.

On this type of column is the earliest eluting peak too early or should I change the gradient.

I would appreciate advice.

Thank you in advance,
Kindest regards,
:lol:

Posted: Fri Dec 05, 2008 12:57 am
by Stryder08
That is pretty early. I would try to push the Rt out further.