Earliest Retention time
Posted: Thu Dec 04, 2008 10:48 pm
Hi all, I am using a Zorbax C18 1.8 um Agilent Eclipse Plus C18 column (2.1 × 50 mm). My earliest analyte eluting peak is at 0.82 min. My run time is 6.5 min.
On this type of column is the earliest eluting peak too early or should I change the gradient.
I would appreciate advice.
Thank you in advance,
Kindest regards,

On this type of column is the earliest eluting peak too early or should I change the gradient.
I would appreciate advice.
Thank you in advance,
Kindest regards,
