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Column Storage Question
Posted: Sun Nov 30, 2008 2:13 am
by WuShock
I was running some hLH/hFSH fractions on my column (Superdex 75 - 10/300 GL) with an isocratic buffer (CH3CN/NH4HCO3) and when I was finished I decided to purge the system and column with milli-q water thinking that the worst that would happen would be I would have to re-equilibrate next time I used it. Manufacturer's website is kind of sketchy on the storage conditions (other than pH and chemical stability). Was this acceptable? Any forseeable problems with long-term storage with just de-ionized micro-filtered water? Link to manufacturer's specs below.
http://www1.gelifesciences.com/aptrix/u ... eid=165422
Posted: Sun Nov 30, 2008 2:16 am
by Consumer Products Guy
I believe I'd make up CH3CN - H2O flush solvent in the same ratio as in your buffer, and wash out all the carbonate, and leave it packed with that.
thanks for reply
Posted: Sun Nov 30, 2008 3:21 am
by WuShock
Thank you kindly sir
Posted: Sun Nov 30, 2008 4:51 pm
by mohan_2008
I would never leave my column sitting in water (even if purified/milli-Q).
If not properly flushed, it can lead to buffer precipitation. Flush with ONLY milliq water to get rid of the salt, completely. Follow this with the ACN/water (without buffer) flush and store in the same.
Long term storage in water can create bacteria, de-stabilize your stationary phase (resulting in longer re-equilibration times, and above all can hydrolyze your stationary phase.
Always aim to store in a 100% ACN if long term. But do not forget to flush with ACN/water prior to running your salted mobile phase.