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HPLC of oils and polyols
Posted: Sat Oct 30, 2004 9:34 pm
by Sasha
Hello, I am looking for appropriate column and mobile phase for analysis of vegetable oils and vegetable oil based polyols. I have used Phase-Riverse and Symmetry Columns with Acetone/Acetonitrile = 70/30 ( and 30/70) mobile phase but separation is very bad. We have Uv and RI detectors. Any help would be appreciated.
Posted: Sun Oct 31, 2004 11:35 am
by maris
Try C30 column.
Posted: Sun Oct 31, 2004 7:16 pm
by Uwe Neue
Sasha:
What is bad about your separation? Peak shape? Resolution/selectivity? Something else? Depending on this, one can make better recommendations...
Posted: Sun Oct 31, 2004 7:19 pm
by Kostas Petritis
I find Sasha's and jschlechte's problem almost identical (although jschlechte explain the problem much better). Maybe these people are from the same lab?