Waters 2695 / Agilent 1100 max column temperature

[c16]

Hi everyone,

We are looking to buy an HPLC system to run some carbohydrate analyses, and the protocol calls for a column temperature of 85°C. I have seen several papers saying they have used this protocol and temperature using a Waters 2695 or Agilent 1100 system, however the manufacturers' specifications indicate temperatures up to 60°C and 80°C, respectively.

Is there any known way to get the column compartments to heat up higher than the manufacturer's specification?

Thank you for your help!
Corentin @ C16 Bio

[scottythree]

Not to my knowledge, but it is highly likely they used an external column compartment (third party) with these systems.

[vofelyp]

Hi
Waters has an external column heater. Max temp is 150 C

[HPLC chemist]

We just used a stirring beaker full of filtered water at 85 Celsius as the mobile phase supply for 'Simple Sugar analysis' (~40 years ago).