Page 1 of 1
Vitamins analysis of human samples
Posted: Fri Nov 21, 2008 2:01 pm
by James Ellis
I am new to HPLC, but I would like to use this technology to assess the levels of water and fat-soluble vitamins from human samples. I would like to be able to identify/quantitate as many vitamins as possible from one method.
Does anyone have a suitable methodology or tips?
Thanks,
James.
Posted: Fri Nov 21, 2008 9:44 pm
by Bruce Hamilton
As always, a good reference book is very helpful.
In this case,
Modern Chromatographic Analysis of Vitamins
Andre P. De Leenheer, Willy E. Lambert, Jan F. Van Bocxlaer
Marcel Dekker. 3rd Ed. ( 2000 ) ISBN: 0-8247-0316-2
This book will provide plenty of literature references, and points out many of the pitfalls of vitamin analysis.
Please keep having fun,
Bruce Hamilton
Posted: Fri Nov 21, 2008 11:09 pm
by Uwe Neue
Look at the Waters website at the following brochure on Atlantis dC18.
http://www.waters.com/webassets/cms/lib ... 0793en.pdf.
On page 7, it shows you a gradient separation of water-soluble and fat-soluble vitamins.
Posted: Tue Dec 02, 2008 5:01 pm
by Bryan Evans
Below is LC-MS/MS application for water soluble vitamins on
Unison UK-C18:
http://www.silvertonesciences.com/files/TI312E.pdf
Posted: Wed Dec 10, 2008 11:38 am
by James Ellis
Thanks everyone. The links and references have been really useful.
James.
Posted: Wed Dec 10, 2008 3:57 pm
by oscarBAL
Hello James; I am starting to read some articles because I am concediring to do my Thesis on a related thesis; I probably I am not so usefull but I advice you put atention to the sample preparation.
you can run fat and waters soluble vitamins in a single run; but this allways going to be limited for your ability to extract as much vitamins as you can in a single step.
This going to be my first experience with this kind of determination;In fact prior to the chromatographic method I am seeking a good sample preparation procedure.
Regards.
Oscar.