Sizeexclu chr,resolutn..18kd intactprotein and 15 kd degradn

[alicee!]

hii..
i need to develop a size exclusion chromatography for resolution of 18 kd intact protein and 15 and 10 kd degraded products of the protein.
any suggestion for columns for the range for which i can expect resolution?
thanks!!

[Uwe Neue]

Protein-Pak 125 from Waters.

[alicee!]

Protein-Pak 125 from Waters.

i have been using this column.. but my 19 kda protein.. and buffer/diluent peak are very near to each other.. no way i can see my 14 and 10 kda protein fragments between them.. any suggestions?? thanks!!

[Uwe Neue]

Several possibilities. The fastest one is to e-mail me a copy of the C-gram. Alternatively, what is the flow rate, what are the retention times of the 19kDa protein and the buffer peak, what is your mobile phase?