Chromatography Forum

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I'm struggling to understand exactly what you're doing. Can you draw a picture of your sampling device? One that indicates where the sorbent is located, how (and how much) of the sample flows into it and where it is in relation to the 10 mL loop? If it's super secret, you probably can't share this information but if you could draw a picture, it'd probably be easier to describe it.

It would be less complicated to push an known amount of the calibration gas through the adsorbent then do the same desorb to column and get area versus amount that way. If you push 10ml of gas through you know how many ug of analyte should be passing through the system for each calibration gas, so you know how many ug passing through the system give a known area count on the MS. From this you take the area counts of the unknown sample versus the calibration curve and find the ug of analyte on the adsorbent, calibrated to 10ml of sample gas.

If you pushed 100ml of sample through the adsorbent then the sample is 10x less concentrated than the calibration gas for the same area counts. For 1L sample size then it is 100x less concentrated than the calibration gas for same area counts.

Sometimes it is better to simplify the process than the calculations.

If you want to calibrate peak area vs analyte quantity then you need to treat your samples and standards in the same way.

Peter