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Stability indicating HPLC method for Minocycline
Posted: Fri Oct 31, 2008 10:19 pm
by Raphy
Can anyone please refer me to a stability indicating HPLC method for testing minocycline and its degradation products? Any publication?
Or any advice?
Thanks!
Posted: Sun Nov 02, 2008 10:28 pm
by mbicking
Use an acidic mobile phase (0.1% formic acid or 0.1% phosphoric acid) with methanol. Detection at about 260 or 360 nm.
A standard reversed phase column may work, but I had better separation with a Zorbax SB-AQ column. Try about 50% methanol. I was able to see the main peak and two main impurities.
Posted: Mon Nov 03, 2008 8:25 am
by yaoguocan
the hplc method for determiation of it has been published in one of chinese journal . the abstract of the method as follows:
mobile phase:water（20mmol/l ammonium acetate pH=6.5）:ACN:MeOH=830:185:5
colum type:C18
wavelength:280nm
Posted: Mon Nov 03, 2008 9:43 pm
by SIELC_Tech
Here is method used by one of our customers for Tetracycline stability method:
http://www.sielc.com/compound_285.html
Your compound is from the same class of compounds and can be analyzed in a similar fshion.
Regards,
Vlad
Posted: Tue Nov 04, 2008 5:03 am
by laserman
This is excerpt from Waters application note book.
http://www.waters.com/webassets/cms/lib ... try168.pdf
Best regards,