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- Posts: 4
- Joined: Tue Jun 24, 2008 12:42 pm
Hi,
we set up a system to separate fat soluble vitamins in complex biological samples. We are working with a Reprosil Gold-C18 (150x3mm; 3µm) column and an ESA Electrochmical detector (Coulochem III). We succeed to seperate Retinol, Retinol acetate, Ergocalciferol, Cholecalciferol, gamma- and alpha-Tocopherol in an isocratic run: MP: 60% MEOH; 30% 1-Prop. and 10% ddH2O. The mobile phase includes 20mM Ac.buffer pH:4.4.
Running an extracted copepod sample we recognized some problems with Resolution in the beginning and end of the run. By adjusting the mobile phase to 70% MeOH; 30% 1-Prop. and 10% ddH2O, with the same Ac.buffer concentration, we could improve the Resolution at the end of the run (20-30min) but lost Resolution in the beginning (5-10min). Do I suffer from a burn out syndrom and loosing control about my mind, or is there a chemical explanation for this?
Thanks in advance for the help! I really appreciate that!
Cheers Norbert
we set up a system to separate fat soluble vitamins in complex biological samples. We are working with a Reprosil Gold-C18 (150x3mm; 3µm) column and an ESA Electrochmical detector (Coulochem III). We succeed to seperate Retinol, Retinol acetate, Ergocalciferol, Cholecalciferol, gamma- and alpha-Tocopherol in an isocratic run: MP: 60% MEOH; 30% 1-Prop. and 10% ddH2O. The mobile phase includes 20mM Ac.buffer pH:4.4.
Running an extracted copepod sample we recognized some problems with Resolution in the beginning and end of the run. By adjusting the mobile phase to 70% MeOH; 30% 1-Prop. and 10% ddH2O, with the same Ac.buffer concentration, we could improve the Resolution at the end of the run (20-30min) but lost Resolution in the beginning (5-10min). Do I suffer from a burn out syndrom and loosing control about my mind, or is there a chemical explanation for this?
Thanks in advance for the help! I really appreciate that!
Cheers Norbert
