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Analyte lost on PL-Sax anion exchange

http://www.chromforum.org/viewtopic.php?t=9386

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Analyte lost on PL-Sax anion exchange

Posted: Tue Oct 21, 2008 12:11 am
by pipettemonkey
I've tried working out an anion exchange method for a natural peptide hormone. I use phosphate buffer in the mobile phase in a gradient from 0 to 1M NaCL. No active ligand is in the flow through, and no active ligand elutes.

I have done this with 10% acetonitrile and 30% 1-propanol in the mobile phase. Nothing has worked.

What's going on here?

Posted: Wed Oct 22, 2008 11:34 am
by goxy43
pH? phosphate concentartion? any organi added to the mobile phase, column temperature, detection? Separation system dimensions? Please specify a bit more closely.
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