urea derivative for gc
Posted: Fri Oct 17, 2008 12:50 pm
Does anyone have a reference for making the dimethylaminomethylene derivative of urea for gc analysis?
Low-abundance plasma and urinary urea enrichments analyzed by gas chromatography/combustion/isotope ratio mass spectrometry
Cornelia C. Metges, Maren Daenzer, Klaus J. Petzke, Angelika Elsner
Journal of Mass Spectrometry
Vol. 37 No: 5 PG: 489-494 YR: 2002
DOI: 10.1002/jms.306
US: http://dx.doi.org/10.1002/jms.306
We report a method for determining plasma und urinary [15N]urea enrichments in an abundance range between 0.37 and 0.52 15N atom% (0-0.15 atom% excess (APE) 15N) using a dimethylaminomethylene derivative. Compared with conventional off-line preparation and 15N analysis of urea, this method requires only small sample volumes (0.5 ml of plasma and 25 µl of urine).