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- Posts: 33
- Joined: Tue May 27, 2008 11:11 am
Our workmates countered a problem: RP-LC is used to separate impurities in an in-process product, and the sample was dissolved in 0.1% sodium pyrosulfite aqueous solution, and HPLC parameters as following:
column: Agilent C18 column;
detection: 210nm, 300nm
temperature:25oC;
inject volume: 10ul;
mobile phase: A: 10mM phosphate buffer(pH2.2) , B: methanol
Gradient program: phase A: 97% to 50%;
phase B: 3% to 50%;
After loading sample, an abnormal chromatogram shows a shoulder peak adhesive to the interest peak(RT 3.2min), and the percetage rises gradually, but the ghost peak exists no longer when sample dissolved in pure water, so we think that sodium pyrosulfite leads some reactions. Please give us some advices. Thanks!
Best regards
Austin[/code]
column: Agilent C18 column;
detection: 210nm, 300nm
temperature:25oC;
inject volume: 10ul;
mobile phase: A: 10mM phosphate buffer(pH2.2) , B: methanol
Gradient program: phase A: 97% to 50%;
phase B: 3% to 50%;
After loading sample, an abnormal chromatogram shows a shoulder peak adhesive to the interest peak(RT 3.2min), and the percetage rises gradually, but the ghost peak exists no longer when sample dissolved in pure water, so we think that sodium pyrosulfite leads some reactions. Please give us some advices. Thanks!
Best regards
Austin[/code]
The God had ever have three apples. Adam was tricked eating up one of them in Eden, and the second dropped from the tree and hit Issac Newton on his head, then what happened on the third one?Interestingly it was bit by Steve Jobs!
