Gentamycin EP analysis
Posted: Tue Oct 14, 2008 6:18 am
Hi,
I'm working for a small pharmaceutical company and we have to perform an analysis on gentamycin sulphate according to the european pharmacopea. This means using an HPLC equiped with a styrene-divinylbenzene copolymer R column with (PLRP-S) with a pore size of 100nm coupled with a PAD.
We used a PLRP-S column from polymer labs with a pore size of 100 Ä, and get some peaks at the right retention time, but very broad peaks.
Then we switched to 1000Ä as this is prescribed, but the peaks are eluting too early (not even half the stated retention time) and also very wide, ugly peaks.
Anyone who has some experience with this method and want's to help me out of this crap?
Ace
I'm working for a small pharmaceutical company and we have to perform an analysis on gentamycin sulphate according to the european pharmacopea. This means using an HPLC equiped with a styrene-divinylbenzene copolymer R column with (PLRP-S) with a pore size of 100nm coupled with a PAD.
We used a PLRP-S column from polymer labs with a pore size of 100 Ä, and get some peaks at the right retention time, but very broad peaks.
Then we switched to 1000Ä as this is prescribed, but the peaks are eluting too early (not even half the stated retention time) and also very wide, ugly peaks.
Anyone who has some experience with this method and want's to help me out of this crap?
Ace