Chromatography Forum

Hello,

I just recently changed a column in a gc-ecd and now the peaks are are really sharp. (which I'm happy about)

but now Chemstation is not recognizing them and doing strange things to analytes which co-elute. Can anyone let me know what integration parameters work best for sharp peaks? (i.e. peak width, threshold, baseline...etc.) I've tried a few options and I can't seem to get it.

Thanks so much.

Hello,

I just recently changed a column in a gc-ecd and now the peaks are are really sharp. (which I'm happy about)

but now Chemstation is not recognizing them and doing strange things to analytes which co-elute. Can anyone let me know what integration parameters work best for sharp peaks? (i.e. peak width, threshold, baseline...etc.) I've tried a few options and I can't seem to get it.

Thanks so much.

You may try to reduce the pw , increase the threshold.

Thanks....seems to be working better

The two most useful integration parameters are Slope Sensitivity and Peak Width.

Increasing the Slope Senstivity will make it harder to find the peak start and stop. As peaks get sharper, they are easier to find, so this parameter can usually be reduced.

Set the Peak Width to the width at half height for the most narrow peak that you are interested in. This is probably what caused most of your problems; the sharper peaks had widths that were much smaller than your setting, which can cause strange results - combining of peaks.