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high temperature reverse phase column??
Posted: Thu Oct 09, 2008 4:16 am
by rick1112
hi
could anyone suggest me reverse phase columns (C8 & C18) which can work at a range of 80oC to 95oC..???
thanks a lot
Posted: Thu Oct 09, 2008 5:53 am
by LC_Master
I think there is a zirchrom column out there which is made out there that goes up to 200 C.
Posted: Thu Oct 09, 2008 6:49 am
by thohry
You can use Zorbax column from Agilent. The StableBond phase C18 can be used up to 90 degrees, C8 and C3 are 80 degrees.
Posted: Thu Oct 09, 2008 1:57 pm
by rhaefe
Hamiltons polymer based PRP-1 column (reversed phased) has been used successfully up to 225°C.
A review article about high temperature HPLC was published in LC-GC in 2004:
Stationary Phases for High-Temperature LC Separations
Yu Yang and Donald R. Lynch, Jr.
Department of Chemistry, East
C a rolina University, Greenville, NC
27858, e-mail
yangy@mail.ecu.edu
LCGC LC COLUMN TECHNOLOGY SUPPLEMENT JUNE 2004, pp34-38
Posted: Fri Oct 10, 2008 5:22 am
by Kelly Johnson
Dear Rick1112:
ZirChrom has a variety of chemistries that are stable throughout the pH range and up to temperatures of 200C. More information is available on our website (
www.zirchrom.com) or if you would like a specific recommendation please feel free to contact me directly
kellyjohnson@zirchrom.com.
Good Luck!
Posted: Sat Oct 11, 2008 6:24 am
by Koen Hollebekkers
We just finished an intensive study towards the stability of stationary phases at high temperature. This will be published soon in the Journal of separation sciences.
Our findings are that the Waters Xbridge is by far the most robust silica based phase column. Columns based on inorganic supports like Zirconia are also stable, but their efficiency is very poor. I would not recommend these columns.
Posted: Mon Oct 13, 2008 7:59 am
by rick1112
hi
just wondering..what happens to stationary phase and bonded phase at high temperature????
Posted: Mon Oct 13, 2008 10:44 am
by Koen Hollebekkers
This totally depend on the conditions of the mobile phase. Under neutral and basic conditions, the silica dissolves. Under acid HT conditions the silica dissolves and the ligands are stripped off. In addition the content of organic modifier also plays a role.
High temperature LC and phase stability are both relative definitions. When is a column stable??
Posted: Mon Oct 13, 2008 4:50 pm
by Kostas Petritis
Porous graphitic carbon columns can also go higher in temperature and pH 1-14 without problem.