sodium pyrosulfite's problem
Posted: Wed Oct 08, 2008 7:55 am
Our workmates countered a problem: RP-LC is used to separate impurities in an in-process product, and the sample was dissolved in 0.1% sodium pyrosulfite aqueous solution, and HPLC parameters as following:
column: Agilent C18 column;
detection: 210nm, 300nm
temperature:25oC;
inject volume: 10ul;
mobile phase: A: 10mM phosphate buffer(pH2.2) , B: methanol
Gradient program: phase A: 97% to 50%;
phase B: 3% to 50%;
After loading sample, an abnormal chromatogram shows a shoulder peak adhesive to the interest peak(RT 3.2min), and the percetage rises gradually, but the ghost peak exists no longer when sample dissolved in pure water, so we think that sodium pyrosulfite leads some reactions. Please give us some advices. Thanks!
Best regards
Austin[/code]
column: Agilent C18 column;
detection: 210nm, 300nm
temperature:25oC;
inject volume: 10ul;
mobile phase: A: 10mM phosphate buffer(pH2.2) , B: methanol
Gradient program: phase A: 97% to 50%;
phase B: 3% to 50%;
After loading sample, an abnormal chromatogram shows a shoulder peak adhesive to the interest peak(RT 3.2min), and the percetage rises gradually, but the ghost peak exists no longer when sample dissolved in pure water, so we think that sodium pyrosulfite leads some reactions. Please give us some advices. Thanks!
Best regards
Austin[/code]