mobile phase

[d.telaro]

Hello,
I'm Daniela. I have an important question for you.
I want to inject uracil dissolved in water in an HPLC system. I would like to use a mobile phase not miscible with water to avoid diluiting uracil. Do you have any suggestion? Can you help me?
Thanks
Daniela

[danko]

Hi Daniela,

Let me understand you right: Should uracil not be soluble in the potential mobile phase? How on earth are you going to elute the uracil once injected on the system?

Best Regards

[JOCAPEN]

Whem the analite is not soluble in mobile phase, there are big problem....Solubility is a first law for HPLC....I bilieve you will need to find another solvents...

[d.telaro]

I don't use a coloumn but a simple restriction and so I don't make the sample eluition.
What do you think about it?

[JOCAPEN]

Your porpose is quantification (HPLC), sample preparation or purification ?

[HW Mueller]

Sounds like you are trying to do flow injection. I have never done it, but it seems that as long as you can have a repeatable flow it should work. Obviously, if you have unknown composition your measurement will be meaningless as there is no separation.
There are masses of information out there on this technique.

[Uwe Neue]

The detector response is going to be very complicated due the fact that most likely you will end up with one or many water bubbles going through the detector cell.

[d.telaro]

I want to make a quantification because I want to test the reproducibility of the system. I really don't want to diluite the uracil. What do you think about it?

Daniela

[danko]

Why don’t you want to dilute the uracil?
Besides, a potential dilution is really a factor that can be ignored in case of “no columnâ€