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mobile phase
Posted: Fri Oct 03, 2008 12:57 pm
by d.telaro
Hello,
I'm Daniela. I have an important question for you.
I want to inject uracil dissolved in water in an HPLC system. I would like to use a mobile phase not miscible with water to avoid diluiting uracil. Do you have any suggestion? Can you help me?
Thanks
Daniela
Posted: Fri Oct 03, 2008 2:03 pm
by danko
Hi Daniela,
Let me understand you right: Should uracil not be soluble in the potential mobile phase? How on earth are you going to elute the uracil once injected on the system?
Best Regards
There is a question ?
Posted: Fri Oct 03, 2008 2:28 pm
by JOCAPEN
Whem the analite is not soluble in mobile phase, there are big problem....Solubility is a first law for HPLC....I bilieve you will need to find another solvents...
no column
Posted: Fri Oct 03, 2008 3:58 pm
by d.telaro
I don't use a coloumn but a simple restriction and so I don't make the sample eluition.
What do you think about it?
sorry....but is not clear...
Posted: Fri Oct 03, 2008 4:38 pm
by JOCAPEN
Your porpose is quantification (HPLC), sample preparation or purification ?
Posted: Sat Oct 04, 2008 8:33 am
by HW Mueller
Sounds like you are trying to do flow injection. I have never done it, but it seems that as long as you can have a repeatable flow it should work. Obviously, if you have unknown composition your measurement will be meaningless as there is no separation.
There are masses of information out there on this technique.
Posted: Mon Oct 06, 2008 3:17 am
by Uwe Neue
The detector response is going to be very complicated due the fact that most likely you will end up with one or many water bubbles going through the detector cell.
diluition problem
Posted: Mon Oct 06, 2008 7:21 am
by d.telaro
I want to make a quantification because I want to test the reproducibility of the system. I really don't want to diluite the uracil. What do you think about it?
Daniela
Posted: Mon Oct 06, 2008 7:49 am
by danko
Why don’t you want to dilute the uracil?
Besides, a potential dilution is really a factor that can be ignored in case of “no columnâ€