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Sedex ELSD model 65 manual
Posted: Thu Oct 02, 2008 4:07 pm
by labstephan
Does anybody have a PDF version (or any other electronic format) of the manual for this ELSD? It would be nice to get a copy, if possible.
Yes, I have asked Sedex in france but approximately 100euro ( ~150USD) for a copy seems to be a lot of money.
I would appreciate if anybody could help.
Stephan
Manual
Posted: Tue Oct 07, 2008 6:29 pm
by chemist91
We have a copy of model 75...let me know if interested
Posted: Tue Oct 07, 2008 10:42 pm
by Kostas Petritis
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The main difference of 65 from the 55 and 75 models is the large LCD.
The software behind it is quite simple so probably you do not need a manual for it. A copy of the 75 will work just fine for you. If you REALLY need a copy of the Sedex 65, I can direct you to a nearby lab in Orleans, France that will probably have a copy...
Posted: Wed Oct 08, 2008 5:41 pm
by labstephan
@ Chemist91: Maria, I received the manual. Thank you very much for your help.
@ Kostas: Kostas, you are right. But I wasn`t aware of it. In the mean time I got some copys about the software of the model 65. I saw that calibration and programmed modi are for quantifying via the detector. I won`t use that feature. I think Chromeleon will do a better job
Due to the fact that this is my "beginning" with ELSD detection it would be nice if anybody knows a good reference on the web. My overall experience in chromatograpy is 16 years but with ELSD I am a newbie
Regards
Stephan
Posted: Wed Oct 08, 2008 5:59 pm
by Kostas Petritis
Once you spend one or two days with it, you will see that it is as easy as using a UV (as long as you respect the volatility requirements of the mobile phase)... and yes chromeleon will do a better job... the only reason I would use the SEDEX software it would be if I want to change the gain during my chromatography...
Posted: Wed Oct 08, 2008 6:25 pm
by labstephan
Oh yes. Thats a good idea. Gain changing could be very interesting since I want to quantify impurities at 0,1% level and having the main compound at approx. 98-99%
Is it true that the response factors for all compounds are nearly the same? Assuming that the volatility is also nearly the same.
My problem is that impurity standards are definitely not available, but structure is known.
Posted: Wed Oct 08, 2008 6:59 pm
by Kostas Petritis
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There are some articles on this, probably from people that were building combinatory libraries. In short yes but not as close as you would like them it to be. Similarity it will depend on many factors, one of them being where they elute during your chromatography (unless you can keep this constant wiht an inverse gradient but at the same time compromising sensitivity). Anyways, I think similar is within 20-40% or so. If your compounds contain nitrogen, a detector will do a better job (but it is harder to use and has a lot of limitations when it comes to additives/solvents that you are allowed to use).