Analysis of zoledronic acid

[Miguel]

Hi there,

Any of you knows how to quantify zoledronic acid (a bisphosphonate with an indol ring at the N-end)?. I have been trying ion-pair RP-LC, but I would like to know if is possible doing it with no ion-pair Chromatography.

Thanks in advance

[sassman]

IC would probably work if you have one available. May or may not give better sensitivity.

[SIELC_Tech]

Here is general method for analysis of "dronic" acids:
http://www.sielc.com/compound_248.html
For zoledronic acid you can use UV with any buffer (pH 2-5)

Contact me if you have questions.

Vlad

[Einar Ponten]

We have numerous of questions about this and similar compounds since these are hydrophilic.

For specific questions please consult our HILIC support <support@sequant.com> or or applications http://www.sequant.com/applications

[Miguel]

Thanks Vlad!!!,

It seems that it is working as you proposed. Thanks a lot

Miguel

[bookoon]

Hi Miguel

Can you please help me with the chromatographic parameters you have used?
I am not sure where to start with mixed mode column.

Thanks

[Vlad Orlovsky]

Start with short column (Primesep SB, 50 mm in length):

1. Inject sample without column and measure peak area, after you inject compound with column peak area should match (for UV or ELSD) sum of all peaks you see in chromatogram

2. Your acid retains mostly by anion-exchange mechanism. In our method we used gradient but only to help elution of nonzwitter-ionic dronic acid. Presence of basic group helps elution of “dronicâ€

[bookoon]

Hi Vlad

Do you think the tailing might reduce if I use a shorter column; 50mm instead of the 150mm I am using currently?