HPLC - ELSD

[ntbhat]

Does anyone have analytical conditions (columns and run time, temperature, flow etc.) for analysis of phosphate, citrate and acetate using ELSD?

Thanks in advance.

[JA]

You only want the whole method then?

I guess your own searches were fruitless and the manufacturers of your HPLC or ELSD equipment are unable to assist.

Four such previous threads you have created soliciting whole method conditions (sample prep and/or chromatographic), providing zero feedback and in some cases not even answering follow-up questions from would be assistants.

Excuse me I'm having a bad evening, but don't forget a bit of give and take.

[Kostas Petritis]

You won't be able to detect acetate with ELSD as it is volatile...

[HW Mueller]

Ja, in my opinion there is no cause for excusing yourself in this.

[ntbhat]

Thank you all for replying. I do not need the whole method but if you can point me in the direction of finding answers to my question, I would appreciate it.

Once again, thanks

[mardexis]

I think your best bet would be an ion exclusion column using 5 mM sulfuric acid mobile phase at a mL/min for a 7.8 mm id coumn and column temperature around 60C with RI detection.

[Einar Ponten]

Try the info at ESA Biosciences

http://www.esainc.com/search/?keyword=hilic