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ascorbic acid
Posted: Sun Jan 13, 2019 12:51 am
by Mohamad
Hello guys,
can someone help me about ran ascorbic acid on GC
Re: ascorbic acid
Posted: Sun Jan 13, 2019 2:38 pm
by rb6banjo
It'll never work. I'd find another way to do it. Firstly, the boiling point is way too high. There are other problems but this is the big one.
Re: ascorbic acid
Posted: Sun Jan 13, 2019 9:32 pm
by Consumer Products Guy
Hello guys,
can someone help me about ran ascorbic acid on GC
Wrong "tool". Would you brush your teeth with a wrench?
Re: ascorbic acid
Posted: Sat Jan 19, 2019 11:58 pm
by Mohamad
thank you for reply. but i have COA from different vendors and all them have GC test and that is the reason make me try to find if there is a method to ran ascorbic acid on GC.
Re: ascorbic acid
Posted: Sun Jan 20, 2019 3:11 pm
by Consumer Products Guy
So contact those suppliers, suppliers will often share QC methods, some can be adapted for use in finished products. But likely GC was used for stuff like residual solvents, etc.
Re: ascorbic acid
Posted: Fri Jan 25, 2019 3:28 pm
by BMU_VMW
What would you like to know about your acid?
e.g. if you want to check for volatile contaminants than headspace sampling of a vial of ascorbic acid should be possible.
Re: ascorbic acid
Posted: Tue Jan 29, 2019 1:52 pm
by MSCHemist
I believe I tried some derivatization methods (ethyl chloroformate) etc haven't found one that worked well. I think silylation may but you definitely need to derivatize as I believe ascorbic has both too high a boiling point and is not thermally stable.
Edit yep silylation with HMDS in acetonitrile.
https://www.sciencedirect.com/science/a ... 3503001919
the complication being it has to be lyophilized because normal evaporation under heat/nitrogen degrades it.
I have a request for it now and I am looking into UV-VIS assays.