membrane protein for GelC/MS/MS
Posted: Fri Oct 22, 2004 1:24 am
Hi,
I am trying to run a membrane fraction using SDS PAGE follow by identification of protein using LC/MS/MS.
One problem is that there are "streaks" appearing due to the insolubility of the membrane samples in the sample loading buffer as such I could not get sharp bands. Would very much appreciate if anyone could suggest how to get good sharp bands?
Thanks and best regards to all.
I am trying to run a membrane fraction using SDS PAGE follow by identification of protein using LC/MS/MS.
One problem is that there are "streaks" appearing due to the insolubility of the membrane samples in the sample loading buffer as such I could not get sharp bands. Would very much appreciate if anyone could suggest how to get good sharp bands?
Thanks and best regards to all.