Chromatography Forum

Posted: **Fri Jan 04, 2019 2:35 pm**

Hello

I have question, can I change mobile phase flow rate eg. from 0,800 ml/min to 0,700 ml/min? will this change be affected by the calculation of the concentration? My calibration curve was provide on 0,800 ml/min.

Thanks

Posted: **Sat Jan 05, 2019 9:42 pm**

You can certainly the flow rate but you WILL have to rerun the calibration at the new flow. Remember that peak area is determined by response multiplied by residence time in the detector cell.

Posted: **Tue Jan 08, 2019 10:05 pm**

Thank you,

OK, I prepare new calibration curve .

I wish you all the best in New Year

Posted: **Wed Jan 09, 2019 1:31 pm**

You can run a standard (made in your lab) or CRM with a flow of 0.7ml/min, and keep the calibration curve with a flow of 0.8 ml/min, and you can see how much it affects the concentration.
It would be best for each flow to have a calibration curve.

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