Relative Response Factor

[ltnguy3]

Hi,
Could any one show me how to calculate relative response factor of known impurities.

Thanks

[DR]

You inject known concentrations of your main peak and the known impurity. Then you calculate response factors for each (area/unit conc). The ratio of these factors is the relative response factor for the known impurity. For example, you inject 1µg/mL each of A and X (X is the impurity). X's response factor is 5 times greater than A's per µg/mL. You then either divide X areas by 5 or multiply them by 0.2 depending on how you set up the ratio (A/X or X/A).

[ltnguy3]

Thank you very much DR,

[Narendra]

How to calculate for relative response factor for unkown impurity?

[ltnguy3]

Any unknown peaks are assumed to have a response factor = 1