Chromatography Forum

I am analyzing about 25 compounds at once. I have one mixture that has the standards for 20 of the compounds, and another mixture that has standards for 5 of the compounds.

I actually know how to use Empower pretty well, but this is killing me. How do I use one of the mixtures to set up the calibration curves for some of the compounds and the other mixture to calibrate the others?

Please let me know if I haven't been clear. If it matters, I am using an internal standard.

Hi Ike

At first You have to create an appropriate processing method:

1, integrate the std injection, set the integration parameters
2, select the component tab in the proc. method
3, select OPTION/FILL TABLE FROM RESULT from the toolbar. The Empower copy the result table to the component table.
4,delete the integrated unknowns
5, type the name of yuor standards. In the fit colunm you can set the cal curve type, the internal std colunm you can set the internal std of each components, the cal curve reference col.determine the other compound that use for quantitation, you can use the relative response factor if it measured. Check the other colunms in this tab for other calculation.

Next step input the std mixture content input.
Select the injection in the project window, Alter sample
The alter sample find the amount button. click it.
find the copy from process method button. The Empower copy the proc. mthod component table. Type the amount of your component, save it. Integrate the std-s.

And let see the f*****g user manual

Don't fill the amount for peaks you don't want to calibrate on, and fill the ones you want to calibrate with.
This should do the trick

Ace

I think my question was misunderstood. Let me describe a simpler, analogous situation.

In each unknown sample, I am analyzing for compounds A and B. The unknowns also have an internal standard added.

I have two different solutions that I need to use to calibrate. Standard solution 1 has a known amount of 'A' and the internal std. Solution 2 has 'B' and the int std. All of the unknowns have A, B and the internal std.

I run into a problem because when the method calibrates on solution 1, it sees no peak (or a very small noise peak) for B.

I think the solution might involve 'levels', but in all the documentation I've been able to find, I can't get a clear explanation of levels.

-fill in the known amount of A and internal standard for Standard solution 1 and leave the amount for component B blank.
-fill in the known amount of B and internal standard for Standard solution 2 and leave the amount for component A blank.

calibrate on this, quantitate your sample, and everything should be fine, no levels needed.

Ace