Chromatography Forum

Hello,

I had a ISTD contamination problem on my GC/MS, I have replaced the liner, septa, cut a small piece off of the injection side of the column, baked out the column, and done solvent rinses, but nothing seems to work. Does anyone have anysuggestions as to what I should try next. The ISTD is 1-Chloro,octane.

Thanks.

Hello!
If you have a autosample change sirnga and change vials.
And then put injector and column high temperature for a night (check the t max).
And we hope good!

Some questions first:

does the contamination appear as a peak, or as background ?

If you run only the GC-MS programme, with no injection, do you still see the contamination ?

If you do a dummy injection (i.e. nothing in the syringe) do you still see the contamination ?

If you inject pure solvent do you still see the contamination, and is it still there with different solvents ?

Peter

Yes the comtamination is a peak.

I am working on the no injection now, if I do an "air" injection yes it is still there.

Yes at solvent injection still shows the peak.

I am having a service tech come out tomorrow to address the issue, so I will let you guys know the out come of the problem for furture reference.

Thanks

It was a durty liner?
Glass wool contaminated?

Sorry I never got back to this I have been slammed at work. Anyway the injection port ended up having to be replaced along with column. All the parts of my autosampler had to be cleaned as well.