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Relative Response Factor
Posted: Mon Oct 18, 2004 1:59 am
by ltnguy3
Hi,
Could any one show me how to calculate relative response factor of known impurities.
Thanks
Posted: Wed Oct 20, 2004 3:36 pm
by DR
You inject known concentrations of your main peak and the known impurity. Then you calculate response factors for each (area/unit conc). The ratio of these factors is the relative response factor for the known impurity. For example, you inject 1µg/mL each of A and X (X is the impurity). X's response factor is 5 times greater than A's per µg/mL. You then either divide X areas by 5 or multiply them by 0.2 depending on how you set up the ratio (A/X or X/A).
Posted: Wed Oct 20, 2004 4:55 pm
by ltnguy3
Thank you very much DR,
Posted: Tue Oct 26, 2004 2:15 pm
by Narendra
How to calculate for relative response factor for unkown impurity?
Posted: Tue Oct 26, 2004 4:12 pm
by ltnguy3
Any unknown peaks are assumed to have a response factor = 1