recovery of proteins on silica size exlusion is low..
Posted: Fri Aug 08, 2008 3:20 am
recovery of proteins on silica size exlusion is low.. i've tried acetate buffer.. phosphate buffer.. both with and without salt.. with and without 10% ethanol.. my protein for lower amounts gives a low protein recovery.. this is on a sillica matrix tsk columns.. any suggestions?? what cud i try?