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recovery of proteins on silica size exlusion is low..

Posted: Fri Aug 08, 2008 3:20 am
by alicee!
recovery of proteins on silica size exlusion is low.. i've tried acetate buffer.. phosphate buffer.. both with and without salt.. with and without 10% ethanol.. my protein for lower amounts gives a low protein recovery.. this is on a sillica matrix tsk columns.. any suggestions?? what cud i try?

Try...

Posted: Fri Dec 18, 2009 10:46 pm
by hplcuser
Sepax SEC, 3um Particle size column: http://www.sepax-tech.com/SEC.php