recovery of proteins on silica size exlusion is low..

[alicee!]

recovery of proteins on silica size exlusion is low.. i've tried acetate buffer.. phosphate buffer.. both with and without salt.. with and without 10% ethanol.. my protein for lower amounts gives a low protein recovery.. this is on a sillica matrix tsk columns.. any suggestions?? what cud i try?

[hplcuser]

Sepax SEC, 3um Particle size column: http://www.sepax-tech.com/SEC.php