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Sizeexclu chr,resolutn..18kd intactprotein and 15 kd degradn

http://www.chromforum.org/viewtopic.php?t=8939

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Sizeexclu chr,resolutn..18kd intactprotein and 15 kd degradn

Posted: Tue Aug 05, 2008 5:06 am
by alicee!
hii..
i need to develop a size exclusion chromatography for resolution of 18 kd intact protein and 15 and 10 kd degraded products of the protein.
any suggestion for columns for the range for which i can expect resolution?
thanks!!

Posted: Wed Aug 06, 2008 10:13 pm
by Uwe Neue
Protein-Pak 125 from Waters.

Posted: Mon Sep 08, 2008 4:54 pm
by alicee!
Protein-Pak 125 from Waters.
i have been using this column.. but my 19 kda protein.. and buffer/diluent peak are very near to each other.. no way i can see my 14 and 10 kda protein fragments between them.. any suggestions?? thanks!!

Posted: Mon Sep 08, 2008 8:25 pm
by Uwe Neue
Several possibilities. The fastest one is to e-mail me a copy of the C-gram. Alternatively, what is the flow rate, what are the retention times of the 19kDa protein and the buffer peak, what is your mobile phase?
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