Chromatography Forum

Hi all, i am a new analytical person to the forum. I am working on a method in which i am using wavelength prog as follows:
0-22 min:283nm,22.1-45 min-210nm,45.1min-283nm.( total run time50min) Reason being the 1st analyte(RT13min) is visible at 283nm and 2nd one(RT30.1min) at 210nm. If such methods are accepted by regulatory authorities. Kindly give suggestions. Thanks in advance

The procedure is acceptable all right.
Most people would monitor both wavelengths simultaneously, utilizing a multi-channel detector or PDA.
Many detectors are capable of monitoring 2 and even 4 different wavelengths at the same time. Maybe yours is capable too?

Best Regards

I have used methods requiring wavelength shifts to support regulatory studies. As long ans the method is correctly validated, there should be no issue.

The biggest problem I had was the detector fialing to perform the change on occasion. however this was immediately obvious on data review.

neel,

just curious, why do you choose switch back to 283nm at 45.1 minutes instead of complet the run at 210nm?.