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HPLC

http://www.chromforum.org/viewtopic.php?t=8886

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HPLC

Posted: Mon Jul 28, 2008 11:19 am
by sharaduj
How much window is allowed for Retention time? is it 5% or 10% with respect to original retention time by HPLC technique.

Posted: Mon Jul 28, 2008 3:46 pm
by tom jupille
That depends entirely on what you are doing. If a simple assay with one component, and no likely interferences, then 15% may be acceptable. On the other hand, if a complex mixture, then 1% or better may be required.
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