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Oxidators
Posted: Thu Oct 14, 2004 7:21 am
by syx
Dear Chromatographers,
Short question:
- What is the effect of oxidators (peroxides) to LC column?
Regards,
SYX
Posted: Thu Oct 14, 2004 10:43 am
by MK
they can retain in the column head and/or free silanols of RP phase and result to baseline humps/interference when flushed out
Posted: Fri Oct 15, 2004 12:24 am
by syx
Is it safe for the column packing?
Posted: Fri Oct 15, 2004 10:13 am
by Yury Zelechonok
I think they are safe.
First, peroxides start to decomposed significantly at elevated temperature usually @ 70C and higher. Second, common RP column has nothing to interact with free radicals. If you use organic modifier the mobile phase is equally good substrate for free radicals and there is much more of it.